You are asked by your research supervisor to prepare 1L of 1M Tris-HCl, pH 6.8. The molecular weight

Question

You are asked by your research supervisor to prepare 1L of 1M Tris-HCl, pH 6.8. The molecular weight of Tris-HCl is 157.59. Show your calculations in preparation of this buffer. The pH of the Tris-HCl solution will be acidic. How will you pH to 6.8?

Answer

add 157.59g Tris-HCL to 900ml water, Bring pH to 6.8 with 1M NaOH using a pH meter, then bring to 1L with water.

A better and cleaner way is to add 121.1g Tris base to ~950ml water, bring pH to 6.8 with 1M HCL.  Note that Tris pKa is 8.1 at 25C so it is a poor buffer at 6.8.

You are asked by your research supervisor to prepare 1L of 1M Tris-HCl, pH 6.8. The molecular weight

Answers (1)