Different proteins may give different results with the Lowry assay, depending on their amino acid content. If each of the following proteins were dissolved in separate tubes at the same concentration, how might the Lowry absorbance readings vary with each protein?
Protein g tryptophan/100g g tyrosine/100g
Collagen 0 1.0
Casein 1.2 6.3
Plasma albumin 0.2 4.7
Haemoglobin (horse) 1.7 3.3
Myosin (rabbit) 2.3 2.4
γ−globulin 2.9 6.8